During the last decades, Nanoparticle Tracking Analysis (NTA) has emerged as a vital and fast characterization technology for biological nanoparticles like Extracellular Vesicles (EVs), Exosomes and Viruses. While classic NTA scatter operation feeds back particle size and total concentration, the fluorescence detection capability (f-NTA) enables the user to gain specific biochemical information. Statistical determination of signal colocalization on low nano scale particles however, is a challenging task for any analytical instrument including flow cytometers and microscopes. A new laser generation paired with ultra fast switching times between fluorescence channels lays the foundation of colocalization nanoparticle tracking analysis (C-NTA) introduced recently by Particle Metrix. For the first time, we report results of colocalization measurements on reference material as well as real biological nanoparticles based on NTA technology on the new ZetaView® PMX-430 QUATT.